Resveratrol Does Not Protect from Ischemia-Induced Acute Kidney Injury in an in Vivo Rat Model

Dr. med. Bienholz, Anja, Mae Pang, Rahel, Dr. med. Rohn, Hana, Univ.-Prof. Dr. med. Rauen, Ursula, Priv.-Doz. Dr. med. Witzke, Oliver, PD Dr. med. Wilde, Benjamin, Priv.-Doz. Dr. rer.nat. Petrat, Frank, Prof. Dr. med. Feldkamp, Thorsten, Prof. Dr. med. Kribben, Andreas

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Background/Aims:
The natural polyphenol resveratrol (RSV) has been shown to ameliorate ischemia/reperfusion (I/R)-induced damage. Therefore, a rat model of I/R-induced AKI equipped with intensive monitoring was utilized to examine direct renal protection by RSV in vivo.
Methods:
AKI was induced by bilateral renal clamping (45 min) followed by reperfusion (3 h). Solvent-free RSV was continuously infused intravenously (0.056 and 0.28 mg/kg) in a total volume of 7 ml/kg/h starting from 30 min before renal clamping. At a mean arterial blood pressure below 70 mmHg for more than 5 min, bolus injections of 0.5 ml 0.9% NaCl solution were administered repetitively (max. 5 ml/kg/h).
Results:
No differences could be found between normoxic control groups with/without RSV. Bilateral renal clamping and subsequent reperfusion caused a progressive rise in creatinine, cystatin C, and CK, a decrease in cellular ATP content and diuresis. Infusion of RSV increased sirtuin 1 expression after ischemia/reperfusion and was associated with decreased blood pressure during ischemia and early reperfusion accompanied by an increased requirement of bolus injections as well as with increased expression of TNFα.
Conclusion:
RSV did not exert protective effects on I/R-induced AKI in the present short-term in vivo rat model. The lack of protection is potentially connected to aggravation of blood pressure instability.
PURL / DOI:
Lesezeichen:
Dokumententyp:
Wissenschaftliche Texte » Artikel, Aufsatz
Fakultät / Institut:
Medizinische Fakultät » Universitätsklinikum Essen » Klinik für Nephrologie
Medizinische Fakultät » Universitätsklinikum Essen » Institut für Physiologische Chemie
Dewey Dezimal-Klassifikation:
600 Technik, Medizin, angewandte Wissenschaften » 610 Medizin und Gesundheit
Stichwörter:
Acute kidney injury, Blood pressure, Ischemia and reperfusion injury, Resveratrol Abstract in Englisch:
Sprache:
Englisch
Kollektion / Status:
E-Publikationen / Dokument veröffentlicht
Dateien geändert am:
02.02.2018
Medientyp:
Text
Lizenz:
Quelle:
Kidney Blood Press Res, 2017;42:1090–1103; published online: December 05, 2017