Gas Chromatography – Differential Mobility Spectrometry and Gas Chromatography – Mass Spectrometry for the detection of coliform bacteria

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The use of miniaturized Gas Chromatography – Differential Mobility Spectrometry (GC-DMS) is shown for the 10 detection and identification of coliform bacteria (including Escherichia coli) grown in five different media: Colilert®-18, glucose broth, M9-medium, tryptophan broth, and tryptic soy broth. After incubation in the different media, headspace 12 containing the volatile compounds were analyzed by the GC-DMS and the results were validated by Gas Chromatography – Mass Spectrometry (GC-MS). Results showed that the GC-DMS and GC-MS were able to detect o-nitrophenol released by coliform bacteria incubated in Colilert®-18. In addition to that, GC-MS was able to detect indole compound released by coliform bacteria grown in all media. Neither GC-DMS nor GC-MS could detect 4-methylumbelliferone from the headspace of E. coli grown in media containing 4-methylumbelliferyl-β-D-glucoronide (MUG) substrate, which was available in Colilert®-18. With the miniaturized GC-DMS being portable and can be operated using ambient pressure, this method offers a potential on-site detection of coliform bacteria.
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Dokumententyp:
Wissenschaftliche Texte » Artikel, Aufsatz
Fakultät / Institut:
Fakultät für Chemie » Analytische Chemie
Dewey Dezimal-Klassifikation:
500 Naturwissenschaften und Mathematik » 540 Chemie
Stichwörter:
Coliform bacteria, Growth media, Biomarker, Gas Chromatography–Differential Mobility Spectrometry, Gas Chromatography–Mass Spectrometry
Sprache:
Englisch
Kollektion / Status:
E-Publikationen / Dokument veröffentlicht
Dateien geändert am:
19.03.2019
Medientyp:
Text
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This is the "Authors Accepted Manuscript" of an article published in: International Journal of Mass Spectrometry, Volume 388, 2015, Pages 17-25; Available online 31 July 2015. The final version may be found at: https://doi.org/10.1016/j.ijms.2015.07.022